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  • Settore di impiego Water Safety Kit

Kit Description


Specific kit for the qualitative, rapid and specific detection of Clostridium perfringens and spores in samples of water for human use, mineral water.

Among the microorganisms considered indicators of fecal pollution that are commonly sought to define the quality of water of different types and intended uses is Clostridium perfringens.

It is present in human, canine and swine feces and is less common or even absent in the feces of other warm-blooded animals.

In wastewater, concentrations of the microorganism can reach values ​​around 105 CFU/100 mL and the reduction of its concentrations, during water purification treatments, can reach 95-98%. In water intended for human consumption, the presence of Clostridium perfringens is rarely reported but its interest in the Water Safety Plans for raw water is increasingly growing.

However, its concentration levels are widely heterogeneous, ranging from <1 CFU/100 mL in uncontaminated waters to values ​​above 3000 CFU/100 mL in waters receiving sewage effluents.

The determination of Cl. perfringens is considered a good supplement for assessing the quality of environmental matrices such as chlorinated water, untreated water, and wastewater. In water intended for human consumption, the parameter can provide information both on the organoleptic and microbiological quality of the water and on the efficiency of the water treatment.

* Rapporti ISTISAN 07/5 ISSN 1123-3117  ** Licenze di brevetto WO 00/28082, WO 01/34790, WO 01/77317, WO 02/24902, WO 01/34838  *** Brevetto per invenzione industriale n. 0001425753 del 09.11.2016

The Kit is composed of

Reagents

To perform rapid DNA extraction and specific gene amplification with LAMP technology

BADGE (RFID)

Contains all information relating to the kit, including the lot number and expiration date

TABLET WITHIN ICGENE APP

With real-time results of Clostridium perfringens detection in analyzed water samples

RAPID BIOMOLECULAR
ANALYSIS

Automatic interpretation of results

The system allows for automatic interpretation of results, real-time visualization, and online availability. Sigmoid curves corresponding to positive (+) samples indicate the presence of Cl. perfringens and spores. Straight lines indicate that the analyzed samples are negative (o) for Cl. perfringens and spores.

FEATURES

  • All reagents included and ready to use
  • Rapid RNA and DNA extraction in minutes at controlled temperature
  • LAMP Technology
  • Tablet with App included to allow results to be read directly
  • Customized solutions for laboratories

BENEFITS

  • Portable system also for field analysis
  • Transportation at room temperature
  • Store reagents between +2° and +8° C
  • Results in 60' maximum
  • Reduction of cross-contamination

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