Details


  • Settore di impiego Water Safety Kit

Kit Description


Specific kit for the qualitative, rapid and specific detection of E.coli O157:H7 in samples of water for human use, mineral water and irrigation water.

Contamination of water by pathogenic Escherichia coli represents a major public health problem, and most pathogenic strains of E. coli isolated in water cause persistent diarrhea. Some of these strains (enterohemorrhagic E. coli) are capable of producing toxins similar to those produced by and particularly aggressive for humans: the toxin Stx-1.

Their spread (EHEC) is assuming particular importance and this subgroup includes the strain E. coli O157:H7 responsible for some important waterborne epidemics. The causes were attributed to the inefficiency of the water purification treatment, during a particularly rainy period that had led to significant soil leaching, and to the presence of sources of environmental contamination (e.g. livestock farms and pastures) that had contributed to the pollution of the water supply source. Due to the methods of waterborne diffusion, there is also keen interest in irrigation and sprouting water. Two distinct investigation kits are available: one for the specific research of toxins, the other for the determination of E. coli O157:H7 responsible for toxin production.

* Patent licenses WO 00/28082, WO 01/34790, WO 01/77317, WO 02/24902, WO 01/34838 ** Industrial invention patent no. 0001425753 dated 09.11.2016  ** Patent for industrial invention no. 0001425753 of 09.11.2016

The Kit is composed of

Reagents

To perform rapid DNA extraction and specific gene amplification with LAMP technology

BADGE (RFID)

Contains all information relating to the kit, including the lot number and expiration date

TABLET WITHIN ICGENE APP

With real-time results of the search for E.coli O157:H7 in the analyzed water samples

RAPID BIOMOLECULAR
ANALYSIS

Automatic interpretation of results

The system allows for automatic interpretation of results, real-time viewing, and online availability. Sigmoid curves corresponding to positive (+) samples indicate the presence of E. coli O157:H7. Straight lines indicate that the analyzed samples are negative (o) for E. coli O157:H7.

FEATURES

  • All reagents included and ready to use
  • Rapid RNA and DNA extraction in minutes at controlled temperature
  • LAMP Technology
  • Tablet with App included to allow results to be read directly
  • Customized solutions for laboratories

BENEFITS

  • Portable system also for field analysis
  • Transportation at room temperature
  • Store reagents between +2° and +8° C
  • Results in 60' maximum
  • Reduction of cross-contamination

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