Details


  • Area of employment Food Safety Kit

Kit Description


Specific kit for the qualitative, rapid and specific detection of Listeria monocytogenes in food matrices.

Listeria is a family of bacteria consisting of ten species.

One of these, Listeria monocytogenes, causes listeriosis, a disease that affects humans and animals.

Listeriosis is one of the most serious foodborne infectious diseases, often severe, with high hospitalization and mortality rates. Listeria is found in soil, plants, and water.

Animals, including cattle, sheep and goats, can also carry the bacterium.

Consumption of contaminated food or feed is the main route of transmission for humans and animals.

Listeria monocytogenes is an environmental microorganism that can contaminate food at all stages of production, handling, and storage: at the distribution level, but also at the domestic level.

Ready-to-eat foods, which, due to their chemical and physical characteristics, support the growth of the microorganism, represent the most at-risk category. These include fresh, soft, and blue cheeses (gorgonzola, Roquefort, Camembert, Robiola, Taleggio, Brie, Brique) and smoked fish (salmon, tuna, swordfish, trout, mackerel, herring). Cooking at temperatures above 65°C kills the bacteria.

However, the Listeria bacterium can be present in various ready-to-eat foods and, unlike many other food-borne bacteria, it tolerates salty environments and low temperatures (between +2°C and 4°C).

Two distinct test kits are available: one for the specific detection of Listeria spp, the other for the determination of Listeria monocytogenes.

* Patent licenses WO 00/28082, WO 01/34790, WO 01/77317, WO 02/24902, WO 01/34838 ** Industrial invention patent no. 0001425753 dated 09.11.2016   ** Patent for industrial invention no. 0001425753 of 09.11.2016

The Kit is composed of

Reagents

To perform rapid DNA extraction and specific gene amplification with LAMP technology

BADGE (RFID)

Contains all information relating to the kit, including the lot number and expiration date

TABLET WITHIN ICGENE APP

Con esito in tempo reale della ricerca di Listeria monocytogenes nei campioni di alimenti analizzati

RAPID BIOMOLECULAR
ANALYSIS

Automatic interpretation of results

The system allows automatic interpretation of the results, real-time visualization and online availability. The sigmoid curves sigmoid curves in correspondence with the positive (+) samples indicate the presence of Listeria monocytogenesThe straight indicate that the analyzed samples are negative (or) for Listeria monocytogenes.

FEATURES

  • All reagents included and ready to use
  • Rapid RNA and DNA extraction in minutes at controlled temperature
  • LAMP Technology
  • Tablet with App included to allow results to be read directly
  • Customized solutions for laboratories

BENEFITS

  • Portable system also for field analysis
  • Transportation at room temperature
  • Store reagents between +2° and +8° C
  • Results in 60' maximum
  • Reduction of cross-contamination

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